The effect of storage temperature and application temperature on the properties of adhesives and resin composites / 口腔疾病防治

@#The properties of adhesives and light-cured resin composites are closely related to the repair of dental defects. Therefore, improving the properties of adhesives and resins composite to increase the success rate of filling has been the focus of research in the field of prosthodontics in recent years. Current studies have confirmed that temperature can change the properties of adhesives and light-cured resin composites, affecting their repair effect. A proper storage temperature ensures the good performance of materials: the self-etching adhesive system should be refrigerated, and the light-cured resin composite should be refrigerated or stored at room temperature according to its composition, proportion and other properties; however, the appropriate storage temperature for the etch-and-rinse adhesive system is not clear. The appropriate application temperature could improve the fluidity, monomer conversion, bonding strength, compressive strength and other properties of the materials to improve the quality of filling restoration. However, there is a wide variety of adhesives and resin composites, and the effect of temperature on each material is different. Thus, it is still necessary to explore the temperature range for material storage, precooling and preheating. Few studies have been performed in vivo, and the clinical restorative effects of adhesives and resin composites stored and used at different temperatures need to be further studied.

Effect of Saliva Decontamination on Bond Strength of 1-step Self-etching Adhesives to Dentin of Primary Posterior Teeth / 대한소아치과학회지

The purpose of this study was to evaluate effect of saliva decontamination procedures on microtensile bond strength (MTBS) of 1-step self-etching adhesives to dentin of primary posterior teeth.63 sound primary-posterior teeth were randomly divided into 3 groups according to different kinds of 1-step self-etching adhesives: Scotchbond™ Universal Adhesive (SBU), All-Bond Universal® (ABU), and Tetric® N Bond Universal (TBU). Each group was randomly categorized into 7 subgroups: (I) application of adhesive without saliva contamination (control); (II – IV) contamination by saliva before photopolymerization; (V – VII) contamination by saliva after photopolymerization; (II, V) decontamination by drying; (III, VI) decontamination by washing and drying; (IV, VII) decontamination by washing, drying, and reapplication of adhesive. All samples were cut into the blocks. At least 15 blocks were tested for each subgroup.For SBU and ABU, the MTBS values of subgroups (I, IV, VII) were significantly higher than those of subgroups (II, III, V, VI). For TBU, the MTBS values of subgroups (I, IV) was significantly higher than those of subgroup (II, III, V, VI).The MTBS of 3 adhesives was reduced by saliva contamination. The adhesive strength on dentin of primary posterior teeth was restored by reapplication of the adhesives after washing and drying.

The effect of adhesive with different unsealing time on the microleakage of adhesive interface / 实用口腔医学杂志

Objective: To investigate the effect of self-etching adhesive with different unsealing time on the microleakage of the adhesive interface between tooth and resin. Methods: Unopened self-etching adhesives, Scotchbond Universal (S) and Xeno V+ (X) were respectively used to adhere Z350 resin for restoration of the prepared occlusal cavities sized 4 mm × 4 mm × 4 mm (n = 40) . The methylene blue staining method was used to observe the microleakage of the adhesive interface between the tooth and filling material at the instant moment, 1, 2 and 3 months after the self-etching adhesives was opened (n = 10) . Results: (1) Increase of microleakage in S group was found with the time span after unsealing, and there was a statistical difference between the instant moment and 3 months after unsealing (P＜0. 05) . (2) There was no significant difference at the 4 test time points in X group (P＞0. 05), although the microleakage value of the samples were increased with the increase of the time after unsealing. (3) The microleakage of X group was greater than that of S group at the instant moment and 1 month after unsealing (P＜0. 05) . Conclusion: Unsealing time may increase the microleakage of the adhesive interface between the tooth and the filling material, the effect varies with the types of the adhesives.

The effects of gingival retraction in the restoration of subgingival non-carious cervical lesions / 实用口腔医学杂志

120 teeth with wedge-shaped defects at subgingival depth of 0 ~2 mm were selected and divided into 3 groups(n =40).Gingival retraction code and EXPASYL gingival retraction paste were used for gingival retraction in the 2 retraction groups,and none retraction was used in the control group.After restoration of the defects,all cases were followed up for 1 year and 2 years.The results were evaluated by modified USPHS criteria.No significant difference was detected for 1 year and 2 year successful rates between the extraction groups(P >0.05).The successful rate in extraction groups was higher than that in the control group(P ＜0.05).

Antibacterial effect of self-etching adhesive systems on Streptococcus mutans

OBJECTIVES: In this study, we evaluated the antibacterial activity of self-etching adhesive systems against Streptococcus mutans using the agar diffusion method. MATERIALS AND METHODS: Three 2-step systems, Clearfil SE Bond (SE, Kuraray), Contax (CT, DMG), and Unifil Bond (UnB, GC), and three 1-step systems, Easy Bond (EB, 3M ESPE), U-Bond (UB, Vericom), and All Bond SE (AB, BISCO) were used. 0.12% chlorhexidine (CHX, Bukwang) and 37% phosphoric acid gel (PA, Vericom) were used as positive controls. RESULTS: The antibacterial activity of CHX and PA was stronger than that of the other groups, except SE. After light activation, the inhibition zone was reduced in the case of all 2-step systems except CT. However, all 1-step systems did not exhibit any inhibition zone upon the light activation. CONCLUSIONS: SE may be better than CT or UnB among the 2-step systems with respect to antibacterial activity, however, 1-step systems do not exhibit any antibacterial activity after light curing.

Nano-resin and self-etching adhesive for bonding repair of anterior tooth defects with vital pulp:A 2-year follow-up / 中国组织工程研究

BACKGROUND:Compared with the conventional composite resin, the 3M Z350 nano-resin has good wear resistance, physical mechanical properties, and polishing, and exerts a lower irritation to the dental pulp. Besides fil ing materials, a reliable tooth-prosthesis bonding interface is necessary for resin bonded repairs. OBJECTIVE:To compare the clinical effects of self-etch bonding Adper Easy One and total-etch bonding Single Bond 2 on nano-resin bonding restoration of the anterior teeth. METHODS:120 anterior teeth with vital pulp, which had defects at the incisal ends and were to be restored with nano resins, were divided into two groups randomly. Two kinds of adhesives, self-etch adhesive and total-etch adhesive, combined with nano-resin were used to restore the teeth. The patients were re-examined immediately, 6 months, 1 year and 2 years after the treatment. The fil ings, teeth and pulps of patients were examined, including whether the prosthesis and tooth color were coordinated, whether the gap between the prosthesis and the teeth were sealed, whether the surface of the prosthesis was intact with no loose, whether the prosthesis and teeth had no staining and secondary caries, whether the condition of the tooth pulp had hot or cold stimulation-induced pain. RESULTS AND CONCLUSION:No significant difference in the fil ing effects was found between the two groups when the patients were re-examined immediately, 6 months and 1 year after the treatment (P>0.05). The pulp lesions of the self-etching group were fewer than those of the total-etch group 2 years after the treatment (Pincomplete restoration and secondary caries, respectively. No statistical y significant differences were found in these four aspects between the two groups (P>0.05). The 2-year fol ow up showed a low incidence of pulp lesions and satisfactory clinical performance after 3M Z350 nano-resin working with self-etching bonding system in the nano-resin fil ing of anterior teeth with vital pulp.

Influence of dentin abrasion methods on the bond strength of self-etching adhesive systems / Influência de métodos de abrasão da dentina na resistência de união de sistemas adesivos autocondicionantes

Objective: The aim of this study was to compare the bond strength of two self-etching adhesive systems with different types of dentin abrasion. Methods: Thirty two sound human molars were selected and had the dentin surface exposed and flattened. Tooth were randomly divided into four experimental conditions, according with the adhesive system [Clearfil SE Bond (SE) and One-Up Bond F(ONE)] and the type of dentin abrasion [conventional diamond bur (D) and ultrasound diamond bur (CVD)]. The adhesives were applied following manufactures’ instructions and light cured composite blocks of 4mm height were placed over dentin. After storage in distilled water for 24 h in 37 ºC, serial cuts were made on mesio-distal and buccal-lingual direction, obtaining specimens with stick format, with cross section of 8mm². The microtensile test was performed at a crosshead speed of 0.5 mm/min and load cell of 10kg, until failure. Data were submitted to ANOVA-2-Way followed by Tukey test (5%). Results: The results (MPa) were: D/ SE: 24.06 ± 8.84, D/ONE:15.03 ± 8.61, CVD/SE: 39.90 ± 8.24 and CVD/ONE:15.03 ±8.61. It can be concluded that the adhesive system Clearfil SE Bond showed higher bond strength related with the abrasion with CVD; the type of dentin abrasion interfered on the performance of Clearfil SE Bond but did not influence the One-Up Bond F results

Objetivo: O objetivo desta pesquisa foi comparar a resistência de união de dois sistemas adesivos autocondicionantes com diferentes métodos de preparo da dentina. Métodos: Foram utilizados 32 molares humanos hígidos extraídos, sendo que a superfície dentinária oclusal foi exposta e planificada. Os dentes foram aleatoriamente divididos em quatro diferentes condições experimentais de acordo com o sistema adesivo: Clearfil SE Bond (SE) ou One- Up Bond F (ONE) e com o método de preparo da dentina: ponta diamantada em alta rotação (D) ou ponta diamantada em ultra-som (CVD). Os adesivos foram aplicados de acordo com as recomendações do fabricante e foram confeccionados blocos de 5mm de altura de resina composta fotopolimerizável, pela técnica incremental. Após estocagem em água destilada por 24 h a 37 ºC, foram realizadas secções seriadas no sentido mésio-distal e vestíbulo-lingual, obtendo-se espécimes em forma de palitos, com secção transversal de aproximadamente 0.8 mm2. Os corpos-de-prova foram submetidos ao ensaio de microtração com velocidade de 0.5mm/min e célula de carga de 10kg. Os dados foram submetidos à análise estatística ANOVA e teste de Tukey (5%). Resultados: Os resultados (MPa) foram: D/SE: 24,06 ± 8,84, D/ONE: 15,03 ± 8,61, CVD/SE: 39,90 ± 8,24 e CVD/ONE:15,03 ± 8,61. Concluiu-se que o sistema adesivo Clearfil SE Bond apresentou superioridade significativa de resistência de união em relação ao preparo com ponta CVD; o método de preparo da dentina interferiu no desempenho do Clearfil SE Bond e não influenciou o desempenho do One-Up Bond F

Resistência de união de sistemas adesivos autocondicionantes em dentina profunda / Bond strength of self-etching adhesive systems to deep dentin

Introdução e objetivo:: Os sistemas adesivos autocondicionantes têm sido largamente utilizados por dentistas e pesquisadores nos procedimentos adesivos de restaurações dentárias. O objetivo deste estudo foi avaliar a resistência de união ao microcisalhamento de sistemas adesivos autocondicionantes em dentina profunda. Material e métodos: Prepararam-se 50 terceiros molares humanos até obtenção de superfícies planas de dentina profunda (2 mm abaixo do limite amelodentinário do sulco central), abrasionadas com lixa de carbeto de silício n.º 600. As amostras foram aleatoriamente divididas em cinco grupos (n = 10), de acordo com os grupos experimentais: 1) adesivo convencional Syntac (controle), 2) adesivo autocondicionante One-Up Bond F Plus (um passo), 3) Hybrid Bond (um passo), 4) AdheSE (dois passos) – unidos ao compósito Tetric Ceram – e 5) Silorane System Adhesive (dois passos), unido ao compósito de baixa contração FiltekSilorane. Os materiais foram aplicados segundo orientações dos fabricantes e com uma matriz Tygon. Os corpos-de-prova foram armazenados em água destilada a 37±2ºC por uma semana. Determinou-se a resistência de união por meio da máquina universal de ensaios Zwick, a uma velocidade de 0,5 mm/minuto. Resultados: Os resultados obtidos em MPa (DP) foram analisados estatisticamente (Anova e teste de Tukey – p < 0,05) e apresentaram os seguintes dados: 2) 25,5 (4,4); 1) 24,9 (5,3); 3) 22,4 (8,1); 4) 21,5 (4,4) e 5) 18,1 (7,2). O teste de Tukey mostrou variações significativas entre os grupos 2 e 5, que não evidenciaram diferenças com os demais grupos testados. Conclusão: O adesivo Silorane e o compósito Filtek Silorane tiveram resistência de união similar à dos demais materiais, porém menor que o One-Up Bond F Plus, quando este foi utilizado com o Tetric Ceram. Os resultados sugerem que, em termos de resistência de união, os adesivos autocondicionantes podem ser uma boa alternativa nos procedimentos restauradores adesivos em dentina profunda.

Introduction and objecttiivee:: The self-etching adhesive systems have been widely used by dentists and researchers in the procedures of adhesive dental restorations. The purpose of this study was to evaluate the microshear bond strength of self-etching adhesive systems to deep dentin.Material and methods: Fifty freshly extracted human third molars were prepared using a diamond saw until obtaining flat surfaces of deep dentin (2 mm below the DEJ of central sulcus), wet-abraded with 600-grit SiC paper. The samples were randomly divided into five groups (n = 10) according to the experimental groups: 1) conventional adhesive Syntac (control), 2) one-step self-etching adhesive One-Up Bond F Plus, 3) onestep self-etching adhesive Hybrid Bond, 4) two-step self-etching adhesive AdheSE – bonded to Tetric Ceram resin composite; and the 5) two-step self-etching adhesive Silorane System Adhesive bonded to low shrinkage resin composite Filtek Silorane. The adhesives and the resin composite were applied according to the manufacturers’ instructions, and using a Tygon tubing mold. The samples were stored in distilled water at 37±2ºC for one week. Microshear bond strengths were determined using a Zwick universal testing machine at a crosshead speed of 0.5 mm/minute. Results: The results obtained in MPa (SD) were statistically analyzed (Anova and Tukey test, p < 0.05), and showed the following results: 2) 25,5 (4,4); 1) 24,9 (5,3); 3) 22,4 (8,1); 4) 21,5 (4,4) and 5) 18,1 (7,2). There was statistically significant variation between groups 2 and 5; which did not show difference to the other groups tested. Conclusion: The Silorane System Adhesive and the low shrinkage resin composite Filtek Silorane showed similar bond strength to Syntac, Hybrid Bond and AdheSE – bonded to Tetric Ceram resin composite; however, being lower than One-Up Bond F Plus bonded to Tetric Ceram. Regarding the bond strength, the results suggest that self-etching adhesive systems can...

Development of nano-fluid movement measuring device and its application to hydrodynamic analysis of dentinal fluid / 대한치과보존학회지

This study was aimed to develop an instrument for real-time measurement of fluid conductance and to investigate the hydrodynamics of dentinal fluid. The instrument consisted of three parts; (1) a glass capillary and a photo sensor for detection of fluid movement, (2) a servo-motor, a lead screw and a ball nut for tracking of fluid movement, (3) a rotary encoder and software for data processing. To observe the blocking effect of dentinal fluid movement, oxalate gel and self-etch adhesive agent were used. BisBlock (Bisco) and Clearfil SE Bond (Kuraray) were applied to the occlusal dentin surface of extracted human teeth. Using this new device, the fluid movement was measured and compared between before and after each agent was applied. The instrument was able to measure dentinal fluid movement with a high resolution (0.196 nL) and the flow occurred with a rate of 0.84 to 15.2 nL/s before treatment. After BisBlock or Clearfil SE Bond was used, the fluid movement was decreased by 39.8 to 89.6%.

Comparative enamel bond strength between light- and dual-cured composites bonded by self-etching adhesives / 대한치과보존학회지

This study compared the microshear bond strength (microSBS) of light-cured and dual-cured composites to enamel bonded with three self-etching adhesives. Crown segments of extracted human molars were cut mesiodistally, and 1 mm thickness of specimen was made. They were assigned to three groups by used adhesives: Xeno group (Xeno III), Adper group (Adper Prompt L-Pop), and AQ group (AQ Bond). Each adhesive was applied to cut enamel surface as per manufacturer's instruction. Light-cured (Filtek Z 250) or dual-cured composite (Luxacore) was bonded to enamel of each specimen using Tygon tube. After storage in distilled water for 24 hours, the bonded specimens were subjected to microSBS testing with a crosshead speed of 1 mm/minute. The mean microSBS (n = 20 for each group) was statistically compared using two-way ANOVA, Tukey HSD, and t test at the 0.05 probability level. The results of this study were as follows; 1. The microSBS of light-cured composite was significantly higher than that of dual-cured composite when same adhesive was applied to enamel. 2. For Z 250, the microSBS of AQ group (9.95 +/- 2.51 MPa) to enamel was significantly higher than that of Adper goup (6.74 +/- 1.80 MPa), but not significantly different with Xeno group (7.73 +/- 2.01 MPa). 3. For Luxacore, the microSBS of Xeno group (5.19 +/- 1.32 MPa) to enamel was significantly higher than that of Adper goup (3.41 +/- 1.19 MPa), but not significantly different with AQ group (4.50 +/- 0.96 MPa).

Clinical Evaluation of Self-etching and Etching Adhesive on Non-carious Cervical Lesions at Two Years / 医学研究杂志

Objective To compare the clinical performance of the self-etching and the phosphoric acid etching adhesive system for restoration of non-carious Class V restorations for a period of two years.Methods There were 37 patients with at least one pairs of similar-sized non-carious cervical lesions participated in the study.120 restorations were placed by one operator,of which 60 with phosphoric acid etching adhesive system(Spectrum TPH,Prime& Bond)and 60 with self-etch(Clearfil AP-X,SE Bond).The restorations were evaluated by ?2 statistics at the baseline,6th,12th and 24th month according to the modified Ryge"s USPHS criteria.Results At two years,106 teeth were reviewed in 33 patients.The retention rates for self-etching adhesive system were 89% and for phosphoric acid etching adhesive system 77%.The percentage of the retention rates of both adhesive systems was not found to be different when calculating the failure rates.No cases in both adhesive systems showed slight marginal discoloration problems.Some cases for each adhesive system had slight color change after the same period.Conclusions The performance of both adhesive systems is excellent during this two-year clinical trial.The self-etch adhesive system exhibites slightly better coloar match than the phosphoric acid etching system.

Influence of additional etching on shear bond strength of self-etching adhesive system to enamel / 대한치과보존학회지

Recently, self-etching adhesive system has been introduced to simplify the clinical bonding procedures. It is less acidic compared to the phosphoric acid, thus there is doubt whether this system has enough bond strength to enamel. The purpose of this study was to investigate the influence of additional etching on the adhesion of resin composite to enamel. Ninety extracted bovine permanent anterior teeth were used. The labial surfaces of the crown were ground with 600-grit abrasive paper under wet condition. The teeth were randomly divided into six groups of 15 teeth each. Clearfil SE Bond(R), Adper(TM) Prompt L-Pop and Tyrian SPE(TM) were used as self-etching primers. Each self-etching primers were applied in both enamel specimens with and without additional etching. For additional etching groups, enamel surface was pretreated with 32% phosphoric acid (UNI-ETCH, Bisco, Inc., Schaumburg, IL, USA). Hybrid resin composite Clearfil AP-X, (Kuraray Co., Ltd., Osaka, Japan) was packed into the mold and light-cured for 40 seconds. Twenty-four hours after storage, the specimens were tested in shear bond strength. The data for each group were subjected to independent t - test at p 0.01). In conclusion, self-etching adhesive system with weak acid seems to have higher bond strength to enamel with additional etching, while self-etching adhesive system with strong acid seems not.

Effect of additional coating of bonding resin on the microtensile bond strength of self-etching adhesives to dentin / 대한치과보존학회지

This study investigated the hypothesis that the dentin bond strength of self-etching adhesive (SEA) might be improved by applying additional layer of bonding resin that might alleviate the pH difference between the SEA and the restorative composite resin. Two SEAs were used in this study; Experimental SEA (Exp, pH: 1.96) and Adper Prompt (AP, 3M ESPE, USA, pH: 1.0). In the control groups, they were applied with two sequential coats. In the experimental groups, after applying the first coat of assigned SEAs, the D/E bonding resin of All-Bond 2 (Bisco Inc., USA, pH: 6.9) was applied as the intermediate adhesive. Z-250 (3M ESPE, USA) composite resin was built-up in order to prepare hourglass-shaped specimens. The microtensile bond strength (MTBS) was measured and the effect of the intermediate layer on the bond strength was analyzed for each SEA using t-test. The fracture mode of each specimen was inspected using stereomicroscope and Field Emission Scanning Electron Microscope (FE-SEM). When D/E bonding resin was applied as the second coat, MTBS was significantly higher than that of the control groups. The incidence of the failure between the adhesive and the composite or between the adhesive and dentin decreased and that of the failure within the adhesive layer increased. According to the results, applying the bonding resin of neutral pH can increase the bond strength of SEAs by alleviating the difference in acidity between the SEA and restorative composite resin.

The change of adaptability change in adhesive systems to dentin substrte according to storage time / 대한치과보존학회지

This study compared the microtensile bond strength (microTBS) and microscopic change of two 2-step and two 1-step self-etching adhesives to dentin according to storage times in distilled water. Occlusal dentin was exposed in 48 human molars. They were divided to four groups by different adhesives: SE Bond group (Clearfil SE Bond), AdheSE group (AdheSE), Adper group (Adper Prompt L-Pop), and Xeno group (Xeno III). Each group was stored in 37degrees C distilled water for 1, 15, and 30 days. Resin-bonded specimens were sectioned into beams and subjected to microTBS testing with a crosshead speed of 1 mm/minute. For SEM observation, one specimen was selected and sectioned in each group after each stroage time. Resin-dentin interface was observed under FE-SEM. In all storage times, mean microTBS of SE group was significantly higher than those of other groups (p < 0.05). There was no significant difference between mean microTBS of SE group and AdheSE group among all storage times, but significant difference between 1- and 30-day storage in mean microTBS of Adper group and Xeno group (p < 0.05). For 1-and 15-day storage, all groups showed the close adaptation between resindentin interfaces. For 30-day storage, resin-dentin interfaces showed wide gap in Adper group and separate pattern in Xeno III group.

Shear bond strength of self-etching adhesives to dentin and sem analysis / 대한치과보존학회지

The purpose of this study was to compare shear bond strength and interfacial pattern of composite bonded to dentin using self-etching adhesive systems. Sixty extracted human molars with exposed occlusal dentin were divided into four groups and bonded with four adhesives and composites: Single Bond/Filtek Z 250(SB), Tyrian SPE-One-Step Plus/Aelitefil(TY), Prompt L-Pop/Filtek Z 250(LP), and One-Up Bond F/Palfique Toughwell(OU). The results of this study were as follows; 1. Shear bond strength for OU was significantly lower than that of other groups(p<0.05). No significant difference was founded among SB, TY, and LP. 2. Failure modes to dentin showed adhesive and mixed for SB, TY, and LP, but them for OU showed adhesive in all spceimens. 3. Dentin-resin interface showed close adaptation for SB, TY, and LP, but it showed gap for OU. 4. The hybrid layers for TY, LP, OU were thinner than that of SB. Adhesive layers were observed between composite and hybrid layer, which were 5 microm thick for TY and 10 microm thick for OU.

Morphological patterns of self-etching primers and self-etching adhesive bonded to tooth structure / 대한치과보존학회지

The purpose of this study was to compare in vitro interfacial relationship of restorations bonded with three self-etching primer adhesives and one self-etching adhesive. Class I cavity preparations were prepared on twenty extracted human molars. Prepared teeth were divided into four groups and restored with four adhesives and composites: Clearfil SE Bond/Clearfil(TM) AP-X (SE), UniFil Bond/UniFil(R) F (UF), FL Bond/Filtek(TM) Z 250 (FL) and Prompt L-Pop/Filtek(TM) Z 250 (LP) After storing in distilled water of room temperature for 24 hours, the specimens were vertically sectioned and decalcified. Morphological patterns between the enamel/dentin and adhesives were observed under SEM. The results of this study were as follows; 1. They showed close adaptation between enamel and SE, UF and FL except for LP. 2. The hybrid layer in dentin was 2 microm thick in SE, 1.5 microm thick in UF, and 0.4 microm in both FL and LP. So, the hybrid layers of SE and UF were slightly thicker than that of FL and LP. 3. The lengths and diameters of resin tags in UF and FL were similar, but those of LP were slightly shorter and slenderer than those of SE. 4. The resin tags were long rod shape in SE, and funnel shape in other groups. Within the limitations of this study, it was concluded that self-etching primer adhesives showed close adaptation on enamel. In addition, the thickness of hybrid layer ranged from 0.4-1.5 microm between adhesives and dentin. The resin tags were long rod or funnel shape, and dimension of them was similar or different among adhesives.

Marginal microleakage of self-etching primer adhesives and a self-etching adhesive / 대한치과보존학회지

This study evaluated the microleakage performance of four self-etcing primer adhesives(Clearfil SE Bond, Clearfil Liner Bond 2, UniFil Bond, and FL Bond) and one self-etching adhesive(Prompt L-Pop). Class V cavity preparations with occlusal margins in enamel and gingival margins in dentin were prepared on both buccal and lingual surfaces of 50 extracted human molar teeth. Prepared teeth were randomly divided into five groups and restored using one of five adhesives and composite resins: Prompt L-Pop/Filtek Z 250(Group 1), Clearfil SE Bond/Clearfil AP-X(Group 2), Clearfil Liner Bond 2/Clearfil AP-X(Group 3), UniFil Bond/UniFil F(Group 4), and FL Bond/Filtek Z 250(Group 5). Following one day storage in room temperature water, the restored teeth were thermocycled for 500 cycles between 5degrees C and 55degrees C. Marginal microleakage was assessed by dye penetration using 2% methylene blue dye. After 24 hours, the teeth were sectioned longitudinally and evaluated for microleakage under steromicroscope. The data were statistically analysed by Kruskal-Wallis Test, Mann-Whitney and Wilcoxon signed ranked tests. The results of this study were as follows; 1. The microleakges at both enamel and dentinal margins were the lowest in group 4, increasing among groups in the following order: group 2, follwed by group 5, follwed by group 1, and the highest in group 3. 2. At the enamel margins, the microleakage of group 3 was significantly higher than those of groups 2, 4 and 5(p0.05).